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- 기준카테고리 : A
제품설명
상세정보
High strength low melting point Agarose
Usage
Immunoelectrophoresis, DNA/RNA recovery after electrophoresis, Double-diffusion Ouchterlony plates to demonstrate antibody-antigen reaction, Preparing large DNA samples for plugs, Enzymatic in-gel manipulation, plants and mamalian cell culture, in-gel manipulation without DNA/RNA extraction, Viral plaque assays, etc.
Specification
Melting temp. : 62℃ (1%) ~ 65℃ (4%)
Gelling temp. : 32℃ (1%) ~ 35℃ (4%)
Gel strength : 570 g/㎠ (2%)
1,540 g/㎠ (3%)
2,460 g/㎠ (4%)
Instruction for Preparation
1.Use a beaker which volume is 3~4 times larger than that of solution.
2.Add buffer and shake until agarose powder mixed well with buffer and check total weight.
Usage
Immunoelectrophoresis, DNA/RNA recovery after electrophoresis, Double-diffusion Ouchterlony plates to demonstrate antibody-antigen reaction, Preparing large DNA samples for plugs, Enzymatic in-gel manipulation, plants and mamalian cell culture, in-gel manipulation without DNA/RNA extraction, Viral plaque assays, etc.
Specification
Melting temp. : 62℃ (1%) ~ 65℃ (4%)
Gelling temp. : 32℃ (1%) ~ 35℃ (4%)
Gel strength : 570 g/㎠ (2%)
1,540 g/㎠ (3%)
2,460 g/㎠ (4%)
EEO (-mr) : 0.05 - 0.08
Moisture : ≤ 7-8 %
Turbidity (1.5%) : ≤ 4 NTU
Sulfate (SO4) : ≤ 0.10%
DNase : None detected
RNase : None detected
Gel Background : very clear
Instruction for Preparation
1.Use a beaker which volume is 3~4 times larger than that of solution.
2.Add buffer and shake until agarose powder mixed well with buffer and check total weight.
3.Heat the beaker by microwave oven on lower power just until bubbles appear and remove it from the microwave oven.
4.Swirl the beaker gently to re-suspend remaining particles.
5.Heat again on lower power until bubbles appear and hold for 30~60 seconds for melt perfectly.
6.Remove the beaker and add hot distilled water until total weight reach the recorded weight.
7.Mix thoroughly and cool down the solution 50~60 ℃ before poring to the cast.
