Thermo Scientific™ Cyto-Plex™ Carboxylated Particles

Description

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.
 

Suspension array analysis is a powerful technique enabling the simultaneous detection and quantitation of multiple analytes with a single sample. Using color coded microspheres with the appropriate dyes, these tests can now be carried out using commonly available flow cytometers.

· Cyto-Plex™ microspheres consist of a highly uniform polystyrene particle with fluorescent intensities which are completely separated from each other
· Carboxylated particles provide different sizes and levels of fluorescent intensities for analysis of multiple analytes
· Use of a single diameter particle for all dye levels saves time by only requiring the development and optimization of one bead chemistry; multiple diameters can be combined to increase the number of analytes measured in one test
· High-density binding sites and low non-specific binding enable coupling with a wide variety of antibodies, nucleic acids and other biomolecules
· Microspheres have a maximum emission at 700 nm and can be excited with either 488 nm or 633 nm lasers
· Emission can be collected in either the PE-Cy5 or APC channels
· Since there is little or no emission in the FITC and PE channels, probes utilizing either of these dyes can be effectively used as reporters

Applications
· Multiplex assay development
· Coupling to antibodies, nucleic acids and other biomolecules